Chap 17 -
Immunization and Immune Testing![]()
I. Immunization


A. Definition and role of immunization.
B. Types of Immunization
1. Live attenuated - measles, mumps, rubella, chickenpox, BCG

2. Inactivated (killed) vaccines - Anthrax, rabies,
3. Bioengineered vaccines - Hepatitis B

4. Toxoids - DT
C. Recommended Vaccination Schedule

D. Boosters
E. Concerns about vaccines
F. Passive Immunization


II. Immune Testing - Prozone and reaction interpretation
A. Precipitation
1. Immunodiffusion

2. Precipitation tests

B. Agglutination
1. Blood testing
2. Hemagglutination as an indicator
C. Complement Fixation
III. Labeled Antibody Tests
A. FTA Fluorescent abs
B. ELISA - Enzyme linked Immuno Sorbent Assays

C. Western Blot - currently used to verify HIV testing

2. Practical applications
http://www.sc2000.net/~czaremba/biology_pictures/monoclonal.GIF

1. Explain and apply the schedule for vaccinations.
2. Differentiate between the
various immune lab tests (percipitation,
agglutination, immunodiffusion, immunofluorescence, RIA and ELISA)
concerning the reaction occurring and their application in
medicine.
3. Describe the reaction occurring in a blood typing test and
interpret the results.
4. Describe
and perform a pregnancy test as a sample of immunologic lab tests.
5.
Perform
an ELISA tutorial online and explain the mechanism for ELISA testing.
Bakersfield College | Kern Community College District |
Janet Fulks
1801 Panorama Dr. - Bakersfield, CA 93305 - (661)395-4381
Date last updated
11/01/2005
ŠJanet Fulks