Chap 17 - Immunization and Immune Testing

I. Immunization

    A. Definition and role of immunization.

    B. Types of Immunization

        1. Live attenuated - measles, mumps, rubella, chickenpox,  BCG

        2. Inactivated (killed) vaccines - Anthrax, rabies,

        3. Bioengineered vaccines - Hepatitis B

        4. Toxoids - DT

 

    C. Recommended Vaccination Schedule

    D. Boosters

 

    E. Concerns about vaccines

 

    F. Passive Immunization

II. Immune Testing - Prozone and reaction interpretation

 

    A. Precipitation

       1. Immunodiffusion

       2. Precipitation tests

    B. Agglutination

        1. Blood testing

        2. Hemagglutination as an indicator

    C. Complement Fixation

III. Labeled Antibody Tests

    A. FTA Fluorescent abs

    B. ELISA - Enzyme linked Immuno Sorbent Assays

 

    C. Western Blot - currently used to verify HIV testing

 

            2. Practical applications


http://www.sc2000.net/~czaremba/biology_pictures/monoclonal.GIF

Latex Agglutination

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Chapter 17 Immunization and Immune Testing

1. Explain and apply the schedule for vaccinations.
2. Differentiate between the various immune lab tests (percipitation, agglutination, immunodiffusion, immunofluorescence, RIA and ELISA) concerning the reaction occurring and their application in medicine.
3. Describe the reaction occurring in a blood typing test and interpret the results.
4.
Describe and perform a pregnancy test as a sample of immunologic lab tests.
5.
 Perform an ELISA tutorial online and explain the mechanism for ELISA testing.

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Bakersfield College | Kern Community College District | Janet Fulks
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Date last updated 11/01/2005
ŠJanet Fulks