Sample Flow Chart
Clean all slides, check slide warmer on      

Broth Culture
¯
loop full on slide
¯
slide warmer
¯
Crystal Violet 10 seconds & rinse
¯
Iodine 20 seconds & rinse
¯
*drips of decolorizer
¯
counterstain safranin 10seconds & rinse
¯
dry on slide warmer

observe on microscope
must use oil

 

  

Stock Culture
  ¯
loop of water on slide
¯
emulsify loop of stock bacteria
¯
slide warmer
¯
Crystal Violet 10 seconds & rinse
¯
Iodine 20 seconds & rinse
¯
*drips of decolorizer
¯
counterstain safranin 10seconds & rinse
¯
dry on slide warmer

observe on microscope
must use oil

                 

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Bakersfield College | Kern Community College District | Janet Fulks
1801 Panorama Dr. - Bakersfield, CA 93305 - (661)395-4011
Date last updated 09/05/2011
©Janet Fulks